Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) ELISA Kit | KTE71446

Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) ELISA Kit | KTE71446 | Gentaur UK, US & Europe Distribution

Application: This Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR1D in samples. An antibody specific for POLR1D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR1D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR1D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR1D bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse DNA-directed RNA polymerases I and III subunit RPAC2 microplate
• Mouse DNA-directed RNA polymerases I and III subunit RPAC2 standard
• Mouse DNA-directed RNA polymerases I and III subunit RPAC2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLR1D. No significant cross-reactivity or interference between Mouse POLR1D and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The peak region of 13q contains POLR1D, a subunit of both RNA polymerases I and III. RNA polymerase I is involved in the production of 18S, 5.8S, and 28S rRNAs, while RNA polymerase III synthesizes small essential RNAs, such as tRNAs, 5S rRNA, and some snRNAs.There is not much information regarding this gene in the literature. POLR1D is overexpressed in 42% of the primary tumors, showing high correlation between expression and copy number. RPA16 cDNA encoding the 16-kDa subunit of mouse RNA polymerase I by a yeast two-hybrid system using mRPA40 as a bait. The deduced amino acid sequence shows 45% identity to the yeast subunit of RNA polymerases I and III, known to associate with AC40, and a local similarity to bacterial alpha subunit.

Alternative Names: POLR1D; FLJ20616; MGC9850; POLR1C; RPA16; RPA9; RPAC2; RPO1-3; OTTHUMP00000018171; RNA polymerase I 16 kDa subunit; polymerase (RNA) I polypeptide D

Search name: POLR1D; FLJ20616; MGC9850; POLR1C; RPA16; RPA9; RPAC2; RPO1-3; OTTHUMP00000018171; RNA polymerase I 16 kDa subunit; polymerase (RNA) I polypeptide D

Tag: POLR1D