Mouse DNA polymerase beta (POLB) ELISA Kit | KTE70715

Mouse DNA polymerase beta (POLB) ELISA Kit | KTE70715 | Gentaur UK, US & Europe Distribution

Application: This Mouse DNA polymerase beta (POLB) ELISA Kit employs a two-site sandwich ELISA to quantitate POLB in samples. An antibody specific for POLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse DNA polymerase beta microplate
• Mouse DNA polymerase beta standard
• Mouse DNA polymerase beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse DNA polymerase beta (POLB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLB. No significant cross-reactivity or interference between Mouse POLB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DNA polymerase beta maintains genome integrity by participating in base excision repair. Over expression of POLB mRNA has been correlated with a number of cancer types whereas deficiencies in POLB results in hypersensitivity to alkylating agents, induced apoptosis and chromosomal breaking therefore it is essential that POLB expression is tightly regulated. POLB gene is upregulated by CREB1 transcription factor binding to the cAMP response element (CRE) present in the promoter of the POLB gene in response to exposure to alkylating agents. POLB gene expression is also regulated at the post transcriptional level as the 3’UTR of the POLB mRNA has been shown to contain three stem-loop structures that influence gene expression.

Alternative Names: POLB; MGC125976; DNA pol beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta

Search name: POLB; MGC125976; DNA pol beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta

Tag: POLB