Mouse DNA primase small subunit (PRIM1) ELISA Kit | KTE70677

Mouse DNA primase small subunit (PRIM1) ELISA Kit | KTE70677 | Gentaur UK, US & Europe Distribution

Application: This Mouse DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1 in samples. An antibody specific for PRIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse DNA primase small subunit microplate
• Mouse DNA primase small subunit standard
• Mouse DNA primase small subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse DNA primase small subunit (PRIM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRIM1. No significant cross-reactivity or interference between Mouse PRIM1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. The DNA polymerase-alpha/primase complex contains 4 subunits: the polymerase-alpha p180 (POLA) and p68 (POLA2) subunits, and the primase p58 (PRIM2A) and p49 (PRIM1) subunits. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis

Alternative Names: PRIM1; MGC12308; p49; DNA primase 1; DNA primase polypeptide 1; DNA primase small subunit; DNA primase subunit 48; primase p49 subunit; primase polypeptide 1; 49kDa

Search name: PRIM1; MGC12308; p49; DNA primase 1; DNA primase polypeptide 1; DNA primase small subunit; DNA primase subunit 48; primase p49 subunit; primase polypeptide 1; 49kDa

Tag: PRIM1