Mouse Dnmt3l Antibody (Center) [APR32994G]

Mouse Dnmt3l Antibody (Center) [APR32994G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse Dnmt3l antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 258-290 amino acids from the Central region of mouse Dnmt3l.

Cellular Localisation: Nucleus.

Molecular Weight: 47993

Clone: Polyclonal

Gene Name: Dnmt3l

Gene ID: 54427

Function: Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (PubMed:11719692, PubMed:15318244, PubMed:15671018, PubMed:24074865) . Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:15671018) . Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:15671018) . Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (By similarity) . Plays a key role in embryonic stem cells and germ cells (PubMed:11719692, PubMed:15318244, PubMed:24074865) . In germ cells, required for the methylation of imprinted loci together with DNMT3A (PubMed:11719692) . In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (PubMed:15318244) . Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (PubMed:24074865) . While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (PubMed:24074865) . Interacts with the EZH2 component of the PRC2/EED- EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (PubMed:24074865) . Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (PubMed:24074865) .

Summary: Tissue Location: Expressed in testis, thymus, ovary, and heart (PubMed:11306809) .

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: DNA (cytosine-5) -methyltransferase 3-like, Dnmt3l