Mouse Doublesex- and mab-3-related transcription factor B1 (DMRTB1) ELISA Kit | KTE71238

Mouse Doublesex- and mab-3-related transcription factor B1 (DMRTB1) ELISA Kit | KTE71238 | Gentaur UK, US & Europe Distribution

Application: This Mouse Doublesex- and mab-3-related transcription factor B1 (DMRTB1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRTB1 in samples. An antibody specific for DMRTB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Doublesex- and mab-3-related transcription factor B1 microplate
• Mouse Doublesex- and mab-3-related transcription factor B1 standard
• Mouse Doublesex- and mab-3-related transcription factor B1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Doublesex- and mab-3-related transcription factor B1 (DMRTB1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRTB1. No significant cross-reactivity or interference between Mouse DMRTB1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: In humans, the DMRT genes encode a large family of transcription factors that are related to the Drosophila doublesex proteins. Expressed primarily in the gonads, the DMRT proteins contain cysteine-rich DNA-binding motifs and are thought to play an important role in sexual development. DMRTB1 (Doublesex- and mab-3-related transcription factor B1) is a 342 amino acid protein that contains one DM DNA-binding domain and belongs to the DMRT family. Localized to the nucleus and expressed in the testis, DMRTB1 may participate in developmental processes and, via its DM domain, may bind to DNA and regulate transcription. DMRTB1 belongs to the DMRT family. Contains 1 DM DNA-binding domain.

Alternative Names: DMRTB1

Search name: DMRTB1

Tag: DMRTB1