Mouse DPYSL2 (Dihydropyrimidinase Like Protein 2) ELISA Kit | ELK7790

Mouse DPYSL2 (Dihydropyrimidinase Like Protein 2) ELISA Kit | ELK7790 | Gentaur UK, US & Europe Distribution

Alternative Names: CRMP2; DHPRP2; DRP-2; DRP2; ULIP2; Unc-33-like phosphoprotein 2; Collapsin response mediator protein 2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 27 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase Like Protein 2 (DPYSL2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dihydropyrimidinase Like Protein 2 (DPYSL2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase Like Protein 2 (DPYSL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidinase Like Protein 2 (DPYSL2) in the samples is then determined by comparing the OD of the samples to the standard curve.