Mouse Early growth response protein 1 (EGR1) ELISA Kit | KTE71394

Mouse Early growth response protein 1 (EGR1) ELISA Kit | KTE71394 | Gentaur UK, US & Europe Distribution

Application: This Mouse Early growth response protein 1 (EGR1) ELISA Kit employs a two-site sandwich ELISA to quantitate EGR1 in samples. An antibody specific for EGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Early growth response protein 1 microplate
• Mouse Early growth response protein 1 standard
• Mouse Early growth response protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Early growth response protein 1 (EGR1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EGR1. No significant cross-reactivity or interference between Mouse EGR1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Zif268 is a mammalian transcription factor that is now officially known as Egr1.It was originally discovered in mouse. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a tumor suppressor gene.The DNA binding domain of Zif268 consists of three zinc finger domains of the Cys2His2 type. The amino acid structure of the Zif268 zinc finger domain is given in this table, using the single letter amino acid code. The fingers 1 to 3 are indicated by f1 - f3. The residues marked 'x' are not part of the zinc fingers, but rather serve to connect them all together.

Alternative Names: EGR1; AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225; early growth response protein 1; nerve growth factor-induced protein A; transcription factor ETR103; zinc finger protein 225

Search name: EGR1; AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225; early growth response protein 1; nerve growth factor-induced protein A; transcription factor ETR103; zinc finger protein 225

Tag: EGR1