Mouse ELA1 (Elastase 1, Pancreatic) ELISA Kit | ELK3328

Mouse ELA1 (Elastase 1, Pancreatic) ELISA Kit | ELK3328 | Gentaur UK, US & Europe Distribution

Alternative Names: PE; CELA1; Chymotrypsin-Like Elastase Family, Member 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 6.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 1, Pancreatic (ELA1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Elastase 1, Pancreatic (ELA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 1, Pancreatic (ELA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 1, Pancreatic (ELA1) in the samples is then determined by comparing the OD of the samples to the standard curve.