Mouse Endoribonuclease Dicer (DICER1) ELISA Kit | KTE71281

Mouse Endoribonuclease Dicer (DICER1) ELISA Kit | KTE71281 | Gentaur UK, US & Europe Distribution

Application: This Mouse Endoribonuclease Dicer (DICER1) ELISA Kit employs a two-site sandwich ELISA to quantitate DICER1 in samples. An antibody specific for DICER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDICER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DICER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DICER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Endoribonuclease Dicer microplate
• Mouse Endoribonuclease Dicer standard
• Mouse Endoribonuclease Dicer detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Endoribonuclease Dicer (DICER1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DICER1. No significant cross-reactivity or interference between Mouse DICER1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity) .

Alternative Names: DICER1; DCR1; Dicer; HERNA; KIAA0928; Dicer1; Dcr-1 homolog; K12H4.8-LIKE; dicer 1; double-stranded RNA-specific endoribonuclease; dicer1; helicase with RNAse motif; helicase-moi

Search name: DICER1; DCR1; Dicer; HERNA; KIAA0928; Dicer1; Dcr-1 homolog; K12H4.8-LIKE; dicer 1; double-stranded RNA-specific endoribonuclease; dicer1; helicase with RNAse motif; helicase-moi

Tag: DICER1