Mouse EPHX4 (Epoxide Hydrolase 4) ELISA Kit | ELK7959

Mouse EPHX4 (Epoxide Hydrolase 4) ELISA Kit | ELK7959 | Gentaur UK, US & Europe Distribution

Alternative Names: ABHD7; EPHXRP; Abhydrolase Domain Containing 7; Epoxide hydrolase-related protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 27.2 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epoxide Hydrolase 4 (EPHX4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epoxide Hydrolase 4 (EPHX4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epoxide Hydrolase 4 (EPHX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epoxide Hydrolase 4 (EPHX4) in the samples is then determined by comparing the OD of the samples to the standard curve.