Mouse ErbB3 (V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit | ELK6845

Mouse ErbB3 (V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit | ELK6845 | Gentaur UK, US & Europe Distribution

Alternative Names: HER3; MDA-BF-1; C-ErbB3; LCCS2; ErbB3-S; P180-ErbB3; P45-SErbB3; P85-SErbB3; Receptor Tyrosine-Protein Kinase ErbB-3; Lethal Congenital Contracture Syndrome 2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.4 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) in the samples is then determined by comparing the OD of the samples to the standard curve.