Mouse F5 (Coagulation Factor V) ELISA Kit | ELK2927

Mouse F5 (Coagulation Factor V) ELISA Kit | ELK2927 | Gentaur UK, US & Europe Distribution

Alternative Names: FV; FVL; PCCF; Proaccelerin; Labile Factor; Factor V Leiden; Accelerator Globulin; Activated protein C cofactor; Proaccelerin, labile factor

Species: Mouse

Assay Type: Sandwich

Sensitivity: 45 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: plasma

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor V (F5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor V (F5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor V (F5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor V (F5) in the samples is then determined by comparing the OD of the samples to the standard curve.