Mouse FATP5 (Fatty Acid Transport Protein 5) ELISA Kit | ELK4637

Mouse FATP5 (Fatty Acid Transport Protein 5) ELISA Kit | ELK4637 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC27A5; ACSB; ACSVL6; FACVL3; VLACSR; BACS; VLCSH2; Fatty Acid Coenzyme A Ligase Very Long Chain 3; Solute Carrier Family 27 Member 5; Bile Acyl CoA Synthetase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Transport Protein 5 (FATP5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fatty Acid Transport Protein 5 (FATP5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Transport Protein 5 (FATP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Transport Protein 5 (FATP5) in the samples is then determined by comparing the OD of the samples to the standard curve.