Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit | AE61127MO

Mouse Fatty acid-binding protein, brain (FABP7) ELISA Kit | AE61127MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: FABP7

Alternative Name: B-FABP; BLBP; DKFZp547J2313; FABPB; MRG; brain lipid binding protein|mammary-derived growth inhibitor-related

Application: ELISA

Range: 62.5-4000 pg/mL

Sensitivity: 25.4 pg/mL

Intra-Assay: ≤5.2%

Inter-Assay: ≤9.4%

Recovery: 1, 08

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate FABP7 in samples. An antibody specific for FABP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFABP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Fatty acid binding protein 7, brain (FABP7; also brain lipid binding protein, BLBP), is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism.FABP7 is expressed, during development, in radial glia by the activation of Notch receptors. Reelin was shown to induce FABP7 expression in neural progenitor cells via Notch-1 activation. According to one study, FABP7 binds DHA with the highest affinity among all of the FABPs.FABP7 maps onto human chromosome 6q22.31, a schizophrenia linkage region corroborated by a meta-analysis..

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .