855

Mouse FcgRI (Receptor I For The Fc Region Of Immunoglobulin G) ELISA Kit | ELK4769

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SKU:
855-ELK4769-GEN
NULL588.00 - NULL704.00

Description

Mouse FcgRI (Receptor I For The Fc Region Of Immunoglobulin G) ELISA Kit | ELK4769 | Gentaur UK, US & Europe Distribution

Alternative Names: CD64; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcgRIA; CD64A; FcgRIB; CD64B; FcgRIC; CD64C; High affinity immunoglobulin gamma Fc receptor I

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.257 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin G (FcgRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) in the samples is then determined by comparing the OD of the samples to the standard curve.

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