Mouse Fibroblast growth factor 11 (FGF11) ELISA Kit | AE61053MO

Mouse Fibroblast growth factor 11 (FGF11) ELISA Kit | AE61053MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: FGF11

Alternative Name: FHF3; FLJ16061; MGC102953; MGC45269; fibroblast growth factor homologous factor 3

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 7.2 pg/mL

Intra-Assay: ≤4.6%

Inter-Assay: ≤7.9%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate FGF11 in samples. An antibody specific for FGF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Fibroblast growth factor 11 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this gene has not yet been determined. The expression pattern of the mouse homolog implies a role in nervous system development.A sequence tagged site (STS), which encompassed 1 exon of FHF3, was derived from chromosome 17 and mapped near the BRCA1 gene which is located on 17q21.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .