Mouse Fibroblast growth factor 11 (FGF11) ELISA Kit | KTE71491

Mouse Fibroblast growth factor 11 (FGF11) ELISA Kit | KTE71491 | Gentaur UK, US & Europe Distribution

Application: This Mouse Fibroblast growth factor 11 (FGF11) ELISA Kit employs a two-site sandwich ELISA to quantitate FGF11 in samples. An antibody specific for FGF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Fibroblast growth factor 11 microplate
• Mouse Fibroblast growth factor 11 standard
• Mouse Fibroblast growth factor 11 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Fibroblast growth factor 11 (FGF11) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FGF11. No significant cross-reactivity or interference between Mouse FGF11 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Fibroblast growth factor 11 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this gene has not yet been determined. The expression pattern of the mouse homolog implies a role in nervous system development.A sequence tagged site (STS), which encompassed 1 exon of FHF3, was derived from chromosome 17 and mapped near the BRCA1 gene which is located on 17q21.

Alternative Names: FGF11; FHF3; FLJ16061; MGC102953; MGC45269; fibroblast growth factor homologous factor 3

Search name: FGF11; FHF3; FLJ16061; MGC102953; MGC45269; fibroblast growth factor homologous factor 3

Tag: FGF11