Mouse FIL1d (Interleukin 1 Delta) ELISA Kit | ELK8559

Mouse FIL1d (Interleukin 1 Delta) ELISA Kit | ELK8559 | Gentaur UK, US & Europe Distribution

Alternative Names: IL1HY1; IL36RN; IL36Ra; IL1RP3; IL1L1; IL1F5; Interleukin 1 Family, Member 5; Interleukin-1 HY1; IL-1 Related Protein 3; Interleukin 36 Receptor Antagonist

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.57 pg/mL

Standard: 100 pg/mL

Detection Range: 1.57-100 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Delta (FIL1d) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Delta (FIL1d) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Delta (FIL1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Delta (FIL1d) in the samples is then determined by comparing the OD of the samples to the standard curve.