Mouse FKBP5 (FK506 Binding Protein 5) ELISA Kit | ELK7803

Mouse FKBP5 (FK506 Binding Protein 5) ELISA Kit | ELK7803 | Gentaur UK, US & Europe Distribution

Alternative Names: P54; FKBP51; FKBP54; Ptg-10; Rotamase; 51 kDa FK506-binding protein; 54 kDa progesterone receptor-associated; Androgen-regulated protein 6; HSP90-binding immunophilin

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.135 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 5 (FKBP5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FK506 Binding Protein 5 (FKBP5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 5 (FKBP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 5 (FKBP5) in the samples is then determined by comparing the OD of the samples to the standard curve.