Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit | ELK1022

Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit | ELK1022 | Gentaur UK, US & Europe Distribution

Alternative Names: FLT3LG; FLT3-LG; Flt3-L; FL; Fms-Related Tyrosine Kinase 3 ligand; SL cytokine

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.33 pg/mL

Standard: 50 pg/mL

Detection Range: 0.79-50 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Immune molecule;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMS Like Tyrosine Kinase 3 Ligand (Flt3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FMS Like Tyrosine Kinase 3 Ligand (Flt3L) in the samples is then determined by comparing the OD of the samples to the standard curve.