Mouse Forkhead box protein C2 (FOXC2) ELISA Kit | KTE71489

Mouse Forkhead box protein C2 (FOXC2) ELISA Kit | KTE71489 | Gentaur UK, US & Europe Distribution

Application: This Mouse Forkhead box protein C2 (FOXC2) ELISA Kit employs a two-site sandwich ELISA to quantitate FOXC2 in samples. An antibody specific for FOXC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFOXC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOXC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOXC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Forkhead box protein C2 microplate
• Mouse Forkhead box protein C2 standard
• Mouse Forkhead box protein C2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Forkhead box protein C2 (FOXC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FOXC2. No significant cross-reactivity or interference between Mouse FOXC2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: FOXC2 is also involved in cancer metastases. Particularly, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. EMT can be induced by a number of genes including Snail, Twist, Goosecoid, and TGF-beta 1. Suppression of FOXC2 expression using shRNA in a highly metastatic breast cancer model blocks their metastatic ability. The nature of these Foxc2-generated responses was consistent with protection against obesity and related symptoms, such as diet-induced insulin resistance. Furthermore, in wildtype mice, Foxc2 mRNA levels were upregulated by high-fat diet, whereas mice with targeted disruption of 1 Foxc2 allele had a decreased interscapular BAT cell mass.

Alternative Names: FOXC2; FKHL14; LD; MFH-1; MFH1; MFH-1;mesenchyme forkhead 1; forkhead box C2; forkhead; Drosophila; homolog-like 14

Search name: FOXC2; FKHL14; LD; MFH-1; MFH1; MFH-1;mesenchyme forkhead 1; forkhead box C2; forkhead; Drosophila; homolog-like 14

Tag: FOXC2