Mouse FUT8 (Fucosyltransferase 8) ELISA Kit | ELK7032

Mouse FUT8 (Fucosyltransferase 8) ELISA Kit | ELK7032 | Gentaur UK, US & Europe Distribution

Alternative Names: GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1, 6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; Alpha- (1, 6) -fucosyltransferase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.127 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosyltransferase 8 (FUT8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fucosyltransferase 8 (FUT8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosyltransferase 8 (FUT8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosyltransferase 8 (FUT8) in the samples is then determined by comparing the OD of the samples to the standard curve.