Mouse gGT1 (Gamma-Glutamyltransferase 1) ELISA Kit | ELK2784

Mouse gGT1 (Gamma-Glutamyltransferase 1) ELISA Kit | ELK2784 | Gentaur UK, US & Europe Distribution

Alternative Names: CD224; G-GT1; GTG; Gamma-glutamyltranspeptidase 1; Glutathione hydrolase 1; Leukotriene-C4 hydrolase;

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.137 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyltransferase 1 (gGT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gamma-Glutamyltransferase 1 (gGT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyltransferase 1 (gGT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-Glutamyltransferase 1 (gGT1) in the samples is then determined by comparing the OD of the samples to the standard curve.