Mouse Glutamic acid decarboxylase autoantibody IgM (GAD-Ab-IgM) ELISA Kit | AE62653MO

Mouse Glutamic acid decarboxylase autoantibody IgM (GAD-Ab-IgM) ELISA Kit | AE62653MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: GAD-Ab-IgM

Alternative Name: N/A

Application: ELISA

Range: Detection antibody

Sensitivity: Request Information

Intra-Assay: ≤5.2%

Inter-Assay: ≤10.3%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Competitive ELISA

Analysis Method : Qualitative

Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAD-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated GAD-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled GAD-Ab-IgM and unlabeled GAD-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GAD-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured.

Product Overview: Glutamate decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD66 wit郀ϛ遰ϛ

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .