Mouse GRb (Glucocorticoid Receptor Beta) ELISA Kit | ELK4311

Mouse GRb (Glucocorticoid Receptor Beta) ELISA Kit | ELK4311 | Gentaur UK, US & Europe Distribution

Alternative Names: GR-B

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Endocrinology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucocorticoid Receptor Beta (GRb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucocorticoid Receptor Beta (GRb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucocorticoid Receptor Beta (GRb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucocorticoid Receptor Beta (GRb) in the samples is then determined by comparing the OD of the samples to the standard curve.