Mouse GUCY1b3 (Guanylate Cyclase 1 Beta 3) ELISA Kit | ELK7769

Mouse GUCY1b3 (Guanylate Cyclase 1 Beta 3) ELISA Kit | ELK7769 | Gentaur UK, US & Europe Distribution

Alternative Names: GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; Soluble guanylate cyclase small subunit; Guanylate cyclase soluble subunit beta-3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase 1 Beta 3 (GUCY1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guanylate Cyclase 1 Beta 3 (GUCY1b3) in the samples is then determined by comparing the OD of the samples to the standard curve.