Mouse GZMK (Granzyme K) ELISA Kit | ELK1805

Mouse GZMK (Granzyme K) ELISA Kit | ELK1805 | Gentaur UK, US & Europe Distribution

Alternative Names: GZM-K; TRYP2; PRSS; Serine Protease, Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme K (GZMK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Granzyme K (GZMK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme K (GZMK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granzyme K (GZMK) in the samples is then determined by comparing the OD of the samples to the standard curve.