Mouse HB (Hemoglobin) ELISA Kit | ELK1354

Mouse HB (Hemoglobin) ELISA Kit | ELK1354 | Gentaur UK, US & Europe Distribution

Alternative Names: Hgb; Haemoglobin; Heterotetramer (αβ) 2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.24 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma and erythrocyte lysates

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin (HB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin (HB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin (HB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin (HB) in the samples is then determined by comparing the OD of the samples to the standard curve.