Mouse HSD17b10 (17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit | ELK6973

Mouse HSD17b10 (17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit | ELK6973 | Gentaur UK, US & Europe Distribution

Alternative Names: 17b-HSD10; ABAD; ERAB; HADH2; HCD2; MHBD; SCHAD; SDR5C1, MRPP2; CAMR; 3-Hydroxyacyl-CoA Dehydrogenase Type-2; AB-Binding Alcohol Dehydrogenase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) in the samples is then determined by comparing the OD of the samples to the standard curve.