Mouse HSPA8 (Heat Shock 70kDa Protein 8) ELISA Kit | ELK1363

Mouse HSPA8 (Heat Shock 70kDa Protein 8) ELISA Kit | ELK1363 | Gentaur UK, US & Europe Distribution

Alternative Names: LAP1; HSC54; HSC70; HSC71; HSP71; HSPA10; NIP71; HSP73; Heat shock cognate 71 kDa protein; Lipopolysaccharide-associated protein 1; LPS-associated protein 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 8 (HSPA8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 8 (HSPA8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 8 (HSPA8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 8 (HSPA8) in the samples is then determined by comparing the OD of the samples to the standard curve.