Mouse IgG2a (Immunoglobulin G2a) ELISA Kit | ELK8143

Mouse IgG2a (Immunoglobulin G2a) ELISA Kit | ELK8143 | Gentaur UK, US & Europe Distribution

Alternative Names: IgG2a

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.075 ug/mL

Standard: 10 ug/mL

Detection Range: 0.16-10 ug/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G2a (IgG2a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G2a (IgG2a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G2a (IgG2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G2a (IgG2a) in the samples is then determined by comparing the OD of the samples to the standard curve.