Mouse IL-6 protein, His tag (Animal-Free) | PRP2112

Mouse IL-6 protein, His tag (Animal-Free) | PRP2112 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines (Animal-Free)

Sequence: Amino acid sequence derived from mouse IL-6 (P08505) (Met 1-Thr211) was expressed with a 6His tag at the C-terminus.

Activity: N/A

protein Lenght: The recombinant mouse IL-6 consists of 193 amino acids and predicts a molecular mass of 21.9 KD. The apparent molecular mass of the mouse IL-6 is approximately 25 KD in SDS-PAGE under reducing conditions due to glycosylation.

Preparation: HEK293 Cells

Purity: >98% as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Molecular Weight: 21.9 KD

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL-6 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Mouse IL-6 protein, His tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.25% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped with blue ice.

Background: IL-6 is a potent inducer of the acute phase response. Rapid production of IL-6 contributes to host defense during infection and tissue injury, but excessive IL-6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglobulin-secreting cells (Probable) . Plays a major role in the differentiation of CD4+ T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL-21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. Required to drive naive CD4+ T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Also required for proliferation of myeloma cells and the survival of plasmablast cells.

Alternative Names: Interleukin-6; IL-6; B-Cell Hybridoma Growth Factor; Interleukin HP-1; Il6; Il-6

Search Names: Interleukin-6; IL-6; B-Cell Hybridoma Growth Factor; Interleukin HP-1; Il6; Il-6

Tag: IL-6