Mouse IL22Ra2 (Interleukin 22 Receptor Alpha 2) ELISA Kit | ELK6517

Mouse IL22Ra2 (Interleukin 22 Receptor Alpha 2) ELISA Kit | ELK6517 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-22RA2;CRF2-S1; IL-22BP; ZcytoR16; Cytokine receptor class-II member 10; Cytokine receptor family type 2, soluble 1; Interleukin-22-binding protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.141 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 22 Receptor Alpha 2 (IL22Ra2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 22 Receptor Alpha 2 (IL22Ra2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 22 Receptor Alpha 2 (IL22Ra2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 22 Receptor Alpha 2 (IL22Ra2) in the samples is then determined by comparing the OD of the samples to the standard curve.