Mouse INHbE (Inhibin Beta E) ELISA Kit | ELK4984

Mouse INHbE (Inhibin Beta E) ELISA Kit | ELK4984 | Gentaur UK, US & Europe Distribution

Alternative Names: INH-bE; Activin beta-E chain

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 1.13 pg/mL

Standard: 250 pg/mL

Detection Range: 3.91-250 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Inhibin Beta E (INHbE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inhibin Beta E (INHbE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibin Beta E (INHbE) in the samples is then determined by comparing the OD of the samples to the standard curve.