Mouse Interleukin-7 (IL-7) ELISA Kit | KTE71482

Mouse Interleukin-7 (IL-7) ELISA Kit | KTE71482 | Gentaur UK, US & Europe Distribution

Application: This Mouse Interleukin-7 (IL-7) ELISA Kit employs a two-site sandwich ELISA to quantitate IL7 in samples. An antibody specific for IL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Interleukin-7 microplate
• Mouse Interleukin-7 standard
• Mouse Interleukin-7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Interleukin-7 (IL-7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL7. No significant cross-reactivity or interference between Mouse IL7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Interleukin 7 is a type I short-chain cytokine belonging to the hematopoietin family. Mouse IL-7 is an approximately 25 kDa monomeric glycoprotein that is synthesized as a 154 amino acid (aa) residue precursor with a 25 aa signal sequence and a 129 aa mature segment. Mature mouse IL-7 has six cysteine residues that form three intrachain disulfide bonds, only one of which is required for bioactivity. Although multiple alternatively spliced isoforms of human IL-7 that differ in their receptor-binding affinities exist, alternatively spliced isoforms of mouse IL-7 have not been reported. Cells known to express IL-7 include Class II MHC positive thymic cortical epithelial cells, follicular dendritic cells, intestinal epithelium, monocytes and keratinocytes, endothelial cells, vascular smooth muscle cells and fibroblasts, and bone marrow stromal cells.

Alternative Names: IL7; IL-7; IL7 nirs variant 6

Search name: IL7; IL-7; IL7 nirs variant 6

Tag: IL7