Mouse Khdrbs1 Antibody (C-term) [APR31820G]

Mouse Khdrbs1 Antibody (C-term) [APR31820G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse Khdrbs1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the C-terminal region of mouse Khdrbs1.

Cellular Localisation: Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q07666}. Membrane Note=Predominantly located in the nucleus but also located partially in the cytoplasm. {ECO:0000250|UniProtKB:Q07666}

Molecular Weight: 48371

Clone: Polyclonal

Gene Name: Khdrbs1 {ECO:0000312|MGI:MGI:893579}

Gene ID: 20218

Function: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE) -containing mRNA with large polyribosomes and translation initiation. May not be involved in the nucleocytoplasmic export of unspliced (CTE) -containing RNA species. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'- [AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly (A) . In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X (S), and of SMN1 (By similarity) . Can regulate CD44 alternative splicing in a Ras pathway-dependent manner. Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (PubMed:12478298, PubMed:22196734, PubMed:24469635) .

Summary: Tissue Location: In adult cerebellum expressed in most neuronal cell populations, specifically in cerebellar granule cells of the internal granular layer, ROR (alpha) -positive Purkinje cells, internal granular layer and molecular layer interneurons (at protein level)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68, Khdrbs1 {ECO:0000312|MGI:MGI:893579}