Mouse LEFTY2 (Left/Right Determination Factor 2) ELISA Kit | ELK6946

Mouse LEFTY2 (Left/Right Determination Factor 2) ELISA Kit | ELK6946 | Gentaur UK, US & Europe Distribution

Alternative Names: TGFB4; EBAF; LEFTA; LEFTYA; Endometrial Bleeding Associated Factor; Left-right determination factor A; Transforming growth factor beta-4

Species: Mouse

Assay Type: Sandwich

Sensitivity: 5.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 2 (LEFTY2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Left/Right Determination Factor 2 (LEFTY2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 2 (LEFTY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 2 (LEFTY2) in the samples is then determined by comparing the OD of the samples to the standard curve.