Mouse LEPR (Leptin Receptor) ELISA Kit | ELK2282

Mouse LEPR (Leptin Receptor) ELISA Kit | ELK2282 | Gentaur UK, US & Europe Distribution

Alternative Names: CD295; LEPR; OBR; LR

Species: Mouse

Assay Type: Sandwich

Sensitivity: 14.5 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Metabolic pathway;Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin Receptor (LEPR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leptin Receptor (LEPR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leptin Receptor (LEPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leptin Receptor (LEPR) in the samples is then determined by comparing the OD of the samples to the standard curve.