Mouse Leucine-rich glioma-inactivated protein 1 (LGI1) ELISA Kit | KTE71179

Mouse Leucine-rich glioma-inactivated protein 1 (LGI1) ELISA Kit | KTE71179 | Gentaur UK, US & Europe Distribution

Application: This Mouse Leucine-rich glioma-inactivated protein 1 (LGI1) ELISA Kit employs a two-site sandwich ELISA to quantitate LGI1 in samples. An antibody specific for LGI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Leucine-rich glioma-inactivated protein 1 microplate
• Mouse Leucine-rich glioma-inactivated protein 1 standard
• Mouse Leucine-rich glioma-inactivated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Leucine-rich glioma-inactivated protein 1 (LGI1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LGI1. No significant cross-reactivity or interference between Mouse LGI1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The deduced 549-amino acid protein contains a N-terminal signal peptide, a putative transmembrane region, and 1 partial and 4 complete leucine-rich repeats (LRRs) . The LRRs are flanked on both sides by cysteine-rich regions, and the N-terminal cysteine-rich region contains a motif conserved in LGIs. LGI3 shares significant similarity with LGI1, LGI2, and LGI4. Semiquantitative PCR detected LGI3 expression in all tissues examined, with highest levels in brain and lung. By in situ hybridization of adult mouse brain, Senechal et al. (2005) showed that Lgi3 was expressed at low levels in most brain regions with high levels in the facial nerve nucleus. Lgi3 was secreted in transfected 293T cells.

Alternative Names: LGI1; EPITEMPIN; EPT; ETL1; IB1099;

Search name: LGI1; EPITEMPIN; EPT; ETL1; IB1099;

Tag: LGI1