Mouse Leukemia inhibitory factor receptor (LIFR) ELISA Kit | KTE71176

Mouse Leukemia inhibitory factor receptor (LIFR) ELISA Kit | KTE71176 | Gentaur UK, US & Europe Distribution

Application: This Mouse Leukemia inhibitory factor receptor (LIFR) ELISA Kit employs a two-site sandwich ELISA to quantitate LIFR in samples. An antibody specific for LIFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Leukemia inhibitory factor receptor microplate
• Mouse Leukemia inhibitory factor receptor standard
• Mouse Leukemia inhibitory factor receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Leukemia inhibitory factor receptor (LIFR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LIFR. No significant cross-reactivity or interference between Mouse LIFR and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LIFR also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, gp130. Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin.

Alternative Names: LIFR; CD118; FLJ98106; FLJ99923; LIF-R; SJS2; STWS; SWS; CD118 antigen; leukemia inhibitory factor receptor

Search name: LIFR; CD118; FLJ98106; FLJ99923; LIF-R; SJS2; STWS; SWS; CD118 antigen; leukemia inhibitory factor receptor

Tag: LIFR