Mouse LNGFR (Low Affinity Nerve Growth Factor Receptor) ELISA Kit | ELK5340

Mouse LNGFR (Low Affinity Nerve Growth Factor Receptor) ELISA Kit | ELK5340 | Gentaur UK, US & Europe Distribution

Alternative Names: CD271; NGFR; TNFRSF16; P75 (NTR) ; Tumor necrosis factor receptor superfamily member 16; P75 Neurotrophin Receptor; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Affinity Nerve Growth Factor Receptor (LNGFR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Low Affinity Nerve Growth Factor Receptor (LNGFR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Affinity Nerve Growth Factor Receptor (LNGFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Affinity Nerve Growth Factor Receptor (LNGFR) in the samples is then determined by comparing the OD of the samples to the standard curve.