Mouse Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit | ELK8890

Mouse Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit | ELK8890 | Gentaur UK, US & Europe Distribution

Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.27pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/ml

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphotactin (Lptn/LTN/XCL1 ) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lymphotactin (Lptn/LTN/XCL1 ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphotactin (Lptn/LTN/XCL1 ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphotactin (Lptn/LTN/XCL1 ) in the samples is then determined by comparing the OD of the samples to the standard curve.