Mouse LY75 (Lymphocyte Antigen 75) ELISA Kit | ELK3532

Mouse LY75 (Lymphocyte Antigen 75) ELISA Kit | ELK3532 | Gentaur UK, US & Europe Distribution

Alternative Names: CD205; LY75; CLEC13B; DEC-205; GP200-MR6; C-type lectin domain family 13 member B

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell Lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 75 (LY75) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lymphocyte Antigen 75 (LY75) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 75 (LY75), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Antigen 75 (LY75) in the samples is then determined by comparing the OD of the samples to the standard curve.