Mouse MAGUK p55 subfamily member 6 (MPP6) ELISA Kit | KTE70968

Mouse MAGUK p55 subfamily member 6 (MPP6) ELISA Kit | KTE70968 | Gentaur UK, US & Europe Distribution

Application: This Mouse MAGUK p55 subfamily member 6 (MPP6) ELISA Kit employs a two-site sandwich ELISA to quantitate MPP6 in samples. An antibody specific for MPP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse MAGUK p55 subfamily member 6 microplate
• Mouse MAGUK p55 subfamily member 6 standard
• Mouse MAGUK p55 subfamily member 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse MAGUK p55 subfamily member 6 (MPP6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MPP6. No significant cross-reactivity or interference between Mouse MPP6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains.

Alternative Names: MPP6; PALS2; VAM-1; VAM1; p55T; MAGUK protein p55T; VELI-associated MAGUK 1; membrane protein; palmitoylated 6; protein associated with Lin7 2

Search name: MPP6; PALS2; VAM-1; VAM1; p55T; MAGUK protein p55T; VELI-associated MAGUK 1; membrane protein; palmitoylated 6; protein associated with Lin7 2

Tag: MPP6