Mouse MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit | KTE71129

Mouse MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit | KTE71129 | Gentaur UK, US & Europe Distribution

Application: This Mouse MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAPK2 in samples. An antibody specific for MAPKAPK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAPK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAPK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAPK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse MAP kinase-activated protein kinase 2 microplate
• Mouse MAP kinase-activated protein kinase 2 standard
• Mouse MAP kinase-activated protein kinase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAPKAPK2. No significant cross-reactivity or interference between Mouse MAPKAPK2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MAP kinase-activated protein kinase 2 is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.The cDNA sequence revealed the following features (in 5-prime to 3-prime order) : a proline-rich region containing 2 putative SH3-binding sites, a kinase catalytic domain, a threonine residue phosphorylated by MAP kinase, and a nuclear localization signal.

Alternative Names: MAPKAPK2; MK2;

Search name: MAPKAPK2; MK2;

Tag: MAPKAPK2