Mouse MIP1a (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit | ELK1116

Mouse MIP1a (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit | ELK1116 | Gentaur UK, US & Europe Distribution

Alternative Names: MIP1 Alpha; CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 5.9 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Alpha (MIP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in the samples is then determined by comparing the OD of the samples to the standard curve.