Mouse MIP1g (Macrophage Inflammatory Protein 1 Gamma) ELISA Kit | ELK7798

Mouse MIP1g (Macrophage Inflammatory Protein 1 Gamma) ELISA Kit | ELK7798 | Gentaur UK, US & Europe Distribution

Alternative Names: CCL9; CCL10; SCYB9; CCF18; MRP2; C-C motif chemokine 9; Macrophage inflammatory protein-related protein 2; Small-inducible cytokine A9

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.3 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity;Human gene deletion

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Gamma (MIP1g) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Gamma (MIP1g) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Gamma (MIP1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Gamma (MIP1g) in the samples is then determined by comparing the OD of the samples to the standard curve.