Mouse Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit | KTE70413

Mouse Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit | KTE70413 | Gentaur UK, US & Europe Distribution

Application: This Mouse Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD1 in samples. An antibody specific for SMAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Mothers against decapentaplegic homolog 1 microplate
• Mouse Mothers against decapentaplegic homolog 1 standard
• Mouse Mothers against decapentaplegic homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SMAD1. No significant cross-reactivity or interference between Mouse SMAD1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The name is a combination of the two. It was found that a mutation in the 'Drosophila' gene, MAD, in the mother, repressed the gene, decapentaplegic, in the embryo. The phrase "Mothers against" was added as a humorous approach since mothers often form organizations opposing various issues eg. This protein mediates the signals of the bone morphogenetic proteins, which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase.

Alternative Names: SMAD1; BSP1; JV4-1; JV41; MADH1; MADR1; MAD; mothers against decapentaplegic homolog 1; Mad-related protein 1; SMAD; mothers against DPP homolog 1; Sma- and Mad-related protein 1; TGF-beta signaling protein 1;

Search name: SMAD1; BSP1; JV4-1; JV41; MADH1; MADR1; MAD; mothers against decapentaplegic homolog 1; Mad-related protein 1; SMAD; mothers against DPP homolog 1; Sma- and Mad-related protein 1; TGF-beta signaling protein 1;

Tag: SMAD1