Mouse MSP (Macrophage Stimulating Protein) ELISA Kit | ELK4676

Mouse MSP (Macrophage Stimulating Protein) ELISA Kit | ELK4676 | Gentaur UK, US & Europe Distribution

Alternative Names: MST1; HGFL; NF15S2; Hepatocyte Growth Factor-Like Protein; Macrophage Stimulating 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 1.47 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Stimulating Protein (MSP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Stimulating Protein (MSP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Stimulating Protein (MSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Stimulating Protein (MSP) in the samples is then determined by comparing the OD of the samples to the standard curve.