Mouse MSTN (Myostatin) ELISA Kit | ELK5280

Mouse MSTN (Myostatin) ELISA Kit | ELK5280 | Gentaur UK, US & Europe Distribution

Alternative Names: GDF8; Growth Differentiation Factor 8

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 253.7 pg/mL

Standard: 50000 pg/mL

Detection Range: 781.25-50000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Cytokine;Metabolic pathway;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Myostatin (MSTN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myostatin (MSTN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myostatin (MSTN) in the samples is then determined by comparing the OD of the samples to the standard curve.